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Research Article |

Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials

Understanding of diffusion of solute particles across the biological partition is very well studied both in vitro and in vivo. Diffusion is the movement of solute molecules by random thermal or Brownian motion across the barriers. Various experimental and mathematical models were employed to study the diffusion characteristics which have also helped to design the experiments and interpretation of data. In the present investigation, we have developed a simple, cost-effective eggshell membrane model for understanding diffusion characteristics. The passive diffusion (in vitro) of sugar molecules through a natural (Chicken egg shell) membrane model was studied. The diffusion coefficient (D), diffusion rate (J), and permeability coefficient (P) in relation to temperature, viscosity of solution, surface area of the membrane, and the molecular size of diffusing molecules have been quantitatively determined. Fick’s first law of diffusion is used for the evaluation of experimental data. All the experiments were carried out at a physiological pH. The diffused solute particles in the donor compartment were measured using UV visible spectroscopy at three different time intervals. Diffusion rate and the permeability coefficient were found to increase with the higher temperature of the solution as well as for the larger surface area of the membrane; however, it decreased with the viscosity and size of the diffusing solute particles. The possible mechanisms and characteristics of the diffusion of the molecules have been elucidated. We conclude that such a model may be useful as a teaching and learning model for diffusion characteristics.

Diffusion, Eggshell Membrane, Sugars, Surface Area, Temperature, Viscosity

APA Style

Shah, P. K. K., Jaiswal, V. D., Kulkarni, A. A., Dongre, P. M. (2023). Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. International Journal of Biochemistry, Biophysics & Molecular Biology, 8(1), 6-11. https://doi.org/10.11648/j.ijbbmb.20230801.12

ACS Style

Shah, P. K. K.; Jaiswal, V. D.; Kulkarni, A. A.; Dongre, P. M. Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. Int. J. Biochem. Biophys. Mol. Biol. 2023, 8(1), 6-11. doi: 10.11648/j.ijbbmb.20230801.12

AMA Style

Shah PKK, Jaiswal VD, Kulkarni AA, Dongre PM. Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. Int J Biochem Biophys Mol Biol. 2023;8(1):6-11. doi: 10.11648/j.ijbbmb.20230801.12

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. David L. N. and Michael C., Lehninger Principles of Biochemistry, New York: W. H. Freeman and Company, 2005.
2. Gerald K., Cell and Molecular Biology Concepts and Experiments, USA: Wiley, 2013.
3. Donald V. and Judith V., Biochemistry, USA: Wiley, 2011.
4. Morton H. F., Principles and Models of Biological Transport, New York: Springer-Verlag, 2008.
5. Bente S., Birger, B. and Carsten U. N., Molecular Biopharmaceutics, Chicago: Pharmaceutical Press, 2010.
6. Ryotaro M., Yasuna I., Takumi Y., Soichiro K., Yuya E., Toshinobu S., Kazuhiko J., Hideo U., and Yasunori M., European Journal of Pharmaceuticla Sciences, 2015, vol. 66, p. 41-49.
7. Boglarka B., Gabor V., Szilvia B., Maria B., Andras K., Balint S., Krisztina T., Piroska S. and Erzsebet C., J. Pharm. Sci., 2016, vol. 105, no. 3 p. 1134-1140.
8. Youn J. J., Jeong-Hyun Y., Nae G. K.., Sun G. P., Seong H. J., Journal of Pharmaceutical Investigation, 2012, vol. 42, p. 271-277.
9. Nys, Y. and Gautron, J., Bioactive Egg Compounds, Berlin, Heidelberg: Springer, 2007.
10. Donald S., Essentials of Physical Chemistry, USA: CRC Press, 2012.
11. Deepika T., Shalaka H., Kaustubh P., Vinod J., Mahesh S., Sivakami S. and Dongre P. M., Current Science, vol. 112, no. 7 p. 1574-1578.
12. Simon, P. C. M., Ultrastructure of hen egg-shell and its physiological interpretation, Netherland: Centre for Agriculture Publishing and Documentation, 1971.
13. Donna L., Maureen M. B. and Tim J. W., J. Synchrotron Rad., 2005, vol. 12, p. 721-726.
14. Upendra D. K., Ravichandran M., Xiaoling L., Indiran P. and Bhaskar J., aaps PharmaSciTech., 2011, vol. 12, no. 2 p. 579-586.
15. Sandip B. T. and Udupa N., Drug Delivery, 2003, vol. 10, p. 161-168.
16. Stanley G. S. and Solomon A. K., The Journal of General Physiology, 1961, vol. 44, p. 1189-1199.
17. Omaima M. S., Silvia F., Daniel W. F., Juliana S., Erika V. Z., Daniel H. F., Mauro F., A. Osama G. and Alessandro G., Lab Chip, 2013, vol. 13, p. 3675-3688.
18. Daniel W., Jason K. C., Jurgen K., Vasco F., Andrea H., John den E., Wim J., Pharm Res., 2015, vol. 32, no. 32 p. 2419-2427.
19. Nicole J. Y. and Marlon J. H., Methods Mol Biol., 2015, vol. 1266, p. 29-53.
20. Gh M.S., Mol Pharm., 2021, vol. 18, p. 2122-2141.
21. Oung S.W., Kremer N., Amara S.B., Zaidi A., Koslowski T., Phys Chem Chem Phys., 2022, vol. 24, p. 14219-14227.
22. Volkova T.V. and Perlovich G.L., Molecules, 2023, vol. 28, p. 389.
23. Michenkova M., Taki S., Blosser M.C., Hwang H.J., Kowatz T., Moss F.J., Occhipinti., Qin X., Sen S., Shinn E., Wang D., Zeise B.S., Zhao P., Malmstadt N., Vahedi-Faridi A., Tajkhorshid E., Boron W.F., Interface Focus, 2021, vol. 11, p. 20200090.
24. Zhang F., Yao Q., Chen X., Zhou H., Zhou M., Li Y., Cheng H., Drug Deliv., 2023, vol. 30, p. 2162626.