Volume 1, Issue 2, December 2016, Page: 46-49
Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria
Bello Ibrahim, Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Abdulsalami Mohammed Sani, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Bulus Timothy, Department of Biochemistry, Kaduna State University, Kaduna, Nigeria
Mukhtar Gambo Lawal, Department of Microbiology, Umaru Musa Yar’adua University, Katsina, Nigeria
Abdullahi Mohammed, Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Abdurrahman Siraj Abubakar, Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Ahmad Mohammad, Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Received: Nov. 18, 2016;       Accepted: Jan. 7, 2017;       Published: Feb. 3, 2017
DOI: 10.11648/j.ijbbmb.20160102.13      View  2411      Downloads  60
Abstract
G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.
Keywords
Glucose-6-phosphate Dehydrogenase, Plasmodium falciparum, Malaria, Children, Katsina State, Nigeria
To cite this article
Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, Abdurrahman Siraj Abubakar, Ahmad Mohammad, Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria, International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 1, No. 2, 2016, pp. 46-49. doi: 10.11648/j.ijbbmb.20160102.13
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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