Volume 4, Issue 1, June 2019, Page: 7-12
"First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "
Rahimatou Yasmine Tiemtoré, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Amana Mètuor-Dabiré, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso; Department of Biochemistry-Microbiology, University of Dédougou, Dédougou, Burkina Faso
Theodora Mahoukèdè Zohoncon, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Yasmine Aminata Bangré, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Serge Sougue, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Jacob Zongo, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Jacques Simpore, Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Received: May 14, 2019;       Accepted: Jun. 17, 2019;       Published: Jun. 28, 2019
DOI: 10.11648/j.ijbbmb.20190401.12      View  716      Downloads  61
Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.
Broad-spectrum Beta-lactamases (ESBL), PER Gene, PCR
To cite this article
Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, Jacob Zongo, Jacques Simpore, "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ", International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 4, No. 1, 2019, pp. 7-12. doi: 10.11648/j.ijbbmb.20190401.12
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
D. L. Monnet, “Consommation d’antibiotiques et résistance bactérienne,” in Annales françaises d’anesthésie et de réanimation, 2000, vol. 19, no. 5, pp. 409–417.
A. Ouedraogo, “Prévalence, circulation et caractérisation des bactéries multirésistantes au Burkina Faso To cite this version : HAL Id : tel-01476152 Spécialité : Biologie Santé,” 2017.
E. Lecaillon et al., “Emergence de Proteus mirabilis et Klebsiella pneumoniae possédant une BLSE: traitement et suivi,” Médecine Mal. Infect., vol. 23, pp. 427–430, 1993.
P. Charlier, “Résistance bactérienne aux B-lactamines,” Medecine/Science, vol. 14, no. lipide II, pp. 544–555, 1998.
D. Vodovar, G. Marcadé, L. Raskine, I. Malissin, and B. Mégarbane, “Entérobactéries productrices de bêta-lactamases à spectre élargi: épidémiologie, facteurs de risque et mesures de prévention,” La Rev. Médecine Interne, vol. 34, no. 11, pp. 687–693, 2013.
K. J. Zongo, A. M. Dabire, L. G. Compaore, I. Sanou, L. Sangare, and J. Simpore, “First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum β - lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina,” vol. 14, no. 14, pp. 1174–1180, 2015.
V. Jarlier, M. Nicolas, G. Fournier, and A. Philippon, “Extended Broad-Spectrum ~ -Lactamases Conferring Transferable Resistance to Newer ~ -Lactam Agents in Enterobacteriaceae : Hospital Prevalence and Susceptibility Patterns,” vol. 10, no. 4, pp. 867–878, 1988.
H. Harbottle, S. Thakur, S. Zhao, and D. G. White, “Genetics of Antimicrobial Resistance,” Anim. Biotechnol., vol. 17, no. 2, pp. 111–124, Dec. 2006.
P. Boerlin and R. J. Reid-Smith, “Antimicrobial resistance: its emergence and transmission,” Anim. Heal. Res. Rev., vol. 9, no. 2, pp. 115–126, 2008.
A. Philippon and G. Arlet, “[Beta-lactamases of Gram negative bacteria: never-ending clockwork!],” Ann. Biol. Clin. (Paris)., vol. 64, no. 1, pp. 37–51, 2006.
P. Feglo et al., “Emergence of a Novel Extended-Spectrum- ␤ -Lactamase (ESBL) - Pathogenic Escherichia coli in Kumasi, Ghana,” vol. 51, no. 2, pp. 728–730, 2013.
A. Muylaert and J. G. Mainil, “Résistances bactériennes aux antibiotiques : les mécanismes et leur « contagiosité »,” pp. 109–123, 2012.
T. Kubo et al., “Corrigendum to ‘Occurrence of infection following prostate biopsy procedures in Japan : Japanese Research Group for Urinary Tract Infection (JRGU) e A multi-center retrospective study’ [J Infect Chemother 20 (2014) 232 e 237],” J. Infect. Chemother., vol. 20, no. 10, p. 660, 2014.
R. Nugent and I. N. Okeke, “Editorial When medicines fail : recommendations for curbing antibiotic resistance,” 2010.
P. Herindrainy, R. Ratovoson, and E. R. Hariniana, “Rectal Carriage of Extended-Spectrum Beta-Lactamase-Producing Gram- Rectal Carriage of Extended-Spectrum Beta-Lactamase- Producing Gram-Negative Bacilli in Community Settings in Madagascar,” no. November 2017, pp. 8–10, 2011.
S. Sirinavin and S. F. Dowell, “Antimicrobial resistance in countries with limited resources: unique challenges and limited alternatives,” Semin. Pediatr. Infect. Dis., vol. 15, no. 2, pp. 94–98, 2004.
C. Zhuo, X. Li, Z. Zong, and N. Zhong, “Epidemic Plasmid Carrying bla CTX-M-15 in Klebsiella penumoniae in China,” vol. 8, no. 1, pp. 1–8, 2013.
A. Mètuor-dabiré, “Caractérisations moléculaire et cinétique des types de β-lactamases à spectre élargi (BLSE) de souches bactériennes collectées au Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle (CHUP-CDG) de Ouagadougou,” Thèse Présentée pour obtenir le grade Docteur l ’ Univ. Ouagadougou, 2014.
P. Nordmann, E. Ronco, T. Naas, C. Duport, Y. Michel-Briand, and R. Labia, “Characterization of a novel extended-spectrum beta-lactamase from Pseudomonas aeruginosa,” Antimicrob. Agents Chemother., vol. 37, no. 5, pp. 962–969, May 1993.
P. Nordmannl, T. Naas, S. De Microbiologie, H. R. Poincare, and F. D. M. Paris-ouest, “Sequence Analysis of PER-1 Extended-Spectrum Comparison with Class A 13-Lactamases,” vol. 38, no. 1, pp. 104–114, 1994.
H. Vahaboglu et al., “Widespread detection of PER-1-type extended-spectrum beta-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: a nationwide multicenter study,” Antimicrob. Agents Chemother., vol. 41, no. 10, pp. 2265–2269, Oct. 1997.
G. Bahar, B. Eraç, A. Mert, and Z. Gülay, “PER-1 Production in a Urinary Isolate of Providencia rettgeri,” J. Chemother., vol. 16, no. 4, pp. 343–346, Aug. 2004.
D. Yong et al., “High prevalence of PER-1 extended-spectrum beta-lactamase-producing Acinetobacter spp. in Korea,” Antimicrob. Agents Chemother., vol. 47, no. 5, pp. 1749–1751, May 2003.
L. Pagani et al., “Multifocal detection of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum beta-lactamase in Northern Italy,” J. Clin. Microbiol., vol. 42, no. 6, pp. 2523–2529, Jun. 2004.
M. PEREIRA et al., “PER-1 Extended-Spectrum β-Lactamase Production in an Alcaligenes faecalis Clinical Isolate Resistant to Expanded-Spectrum Cephalosporins and Monobactams from a Hospital in Northern Italy,” Microb. Drug Resist. vol. 6, no. 1, pp. 85–90, Jan. 2000.
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