Volume 5, Issue 1, June 2020, Page: 8-17
Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia
Shibiru Eticha, Department of Chemistry, Wollega University, Nekemte, Ethiopia
Tesfa Bedassa, Department of Chemistry, Wollega University, Nekemte, Ethiopia
Received: May 15, 2020;       Accepted: Jun. 1, 2020;       Published: Aug. 27, 2020
DOI: 10.11648/j.ijbbmb.20200501.12      View  49      Downloads  49
In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R2 = 0.9999 and R2 = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.
Coffee Samples, Caffeine, UV-Vis Spectrophotometry, High Performance Liquid Chromatography, Horro Guduru Wollega, East Wollega
To cite this article
Shibiru Eticha, Tesfa Bedassa, Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia, International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 5, No. 1, 2020, pp. 8-17. doi: 10.11648/j.ijbbmb.20200501.12
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Murthy, P. S. & Naidu, M. M. Sustainable management of coffee industry by-products and value addition. Resources, Conservation and Recycling, 2012, 66, 45–58.
Yonas Belete, Bayetta Belachew & Chemeda Fininsa. Evaluation of bean qualities of indigenous Arabica Coffee genotypes across different environments. Journal of Plant Breeding and Crop Science, 2014, 6, 135-143.
Mussattoa, I., Livia, M., Carneirob, P. A. & Teixeira, A. A study on chemical constituents and sugar extraction from spent coffee grounds. Carbohydrate Polymers, 2011, 83, 368–374.
Gomez-Rui, J. N., David, S. L. & Jennifer, M. In vitro antioxidant activity of coffee compounds and their metabolites. Journal of Agriculture and Food Chemistry, 2007, 55, 6962-6969.
Nuhu, A. Bioactive micronutrients in coffee: Recent analytical approaches for characterization and quantification. ISRN Nutrition. Retrieved from http://dx.doi.org/10.1155/2014/384230, 2014.
Alonso-Salces, M., Francesca, S., Fabiano, R. & Berger, K. Botanical and geographical characterization of green coffee (Coffea arabica and Coffea canephora): Chemometric evaluation of phenolic and methylxanthine contents. Journal of Agriculture and Food Chemistry, 2009, 57, 4224–4235.
Shishov, A., Volodina, N., Nechaeva, D., Gagarinova, S., & Bulatov, A. An automated homogeneous liquid-liquid microextraction based on deep eutectic solvent for the HPLC- UV determination of caffeine in beverages. Microchemical Journal, 2019, 144, 469-473.
Vuong, V. & Roach, D. Caffeine in green tea. Its removal and isolation. Separation & Purification Reviews, 2014, 43, 155-174.
Oba, S., Nagata, C., Nakamura, K., Fujii, K., Kawachi, T., Takatsuka, N. & Hiroyuki S. Consumption of coffee, green tea, oolong tea, black tea, chocolate snacks and the caffeine content in relation to risk of diabetes in Japanese men and women. British Journal of Nutrition, 2010, 103, 453–459.
Bhupathiraju, S N., Pan, A., Vasanti, S., Manson, E., Willett, C., Dam, M. & Frank, B Caffeinated and caffeine-free beverages and risk of type 2 diabetes. American Journal of Clinical Nutrition, 2013, 97, 163–174.
Mazzafera, P. & Silvarolla, M. Caffeine content variation in single green Arabica coffee seeds. Seed Science Research, 2010, 20, 163-167.
Tello, J., Viguera, M. & Calvo, L. Extraction of caffeine from Robusta coffee (Coffea canephora vs Robusta) husks using supercritical carbon dioxide. The Journal of Supercritical Fluids, 2011, 59, 53–60.
Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A. & Feeley, M. Effects of caffeine on human health. Food Additives and Contaminants, 2003, 20, 1–30.
Tsvetkova, B. G., Kostova, B. D., Rachev, D. R., Peikova, L. T. & Pencheva, I. P. HPLC assay and stability studies of tablets containing paracetamol and caffeine. International Journal of Pharmaceutical Sciences Review and Research, 2013, 18, 138-142.
Rodrigues, N. P. & Bragagnolo, N. Identification and quantification of bioactive compounds in coffee brews by HPLC–DAD–MSn. Journal of Food Composition and Analysis, 2013, 32, 105–115.
Magalhaes, L. M., Sandia, M., Marcela, S., Joa, A. L. & Pascoa, N. M. Rapid assessment of bioactive phenolics and methylxanthines in spent coffee grounds by FT-NIRspectroscopy. Talanta, 2016, 143, 460–467.
Vichare, V., Mujgond, P., Tambe, V. & Dhole, S. N. Simultaneous spectrophotometric determination of paracetamol and caffeine in tablet formulation. International Journal of PharmTech Research, 2010, 2, 2512-2516.
Tautua, A. W., Bamidele, M. & Diepreye, E. R. Ultra-violet spectrophotometric determination of caffeine in soft and energy drinks available in Yenagoa, Nigeria. Advance Journal of Food Science and Technology, 2014, 6, 155-158.
Rotko, T. K. & Bęczkowska, I. Nafion covered lead film electrode for the voltammetric determination of caffeine in beverage samples and pharmaceutical formulations. Food Chemistry, 2015, 172, 24-29.
Maidon, B., Atikah, O. & Hermen, S. Study of various solvents for caffeine determination using UV-Vis Spectrophotometeric method. Journal of Applied Sciences Research, 2012, 8, 2439-2442.
Navarra, G., Moschetti, M., Guarrasi, V., Mangione, M. R., Militello, V. & Leone. M. Simultaneous determination of caffeine and chlorogenic acids in green coffee by UV-Vis Spectroscopy. Journal of chemistry. Retrieved from https://doi.org/10.1155/2017/6435086, 2017.
Mulu Hagos, Mesfin Redi-Abshiro, Bhagwan Singh Chandravanshi, Estifanos Ele Ahmed M. Mohammed & Hassen Mamo. The correlation between caffeine contents of green coffee beans and altitudes of the coffee plants grown in South West Ethiopia. Bulletin Chemical Society of Ethiopia, 2018, 32, 13-25.
Abebe Belay, Kassahun Ture, Mesfin Redi & Araya Asfaw. Measurement of caffeine in coffee beans with UV-Vis spectrophotometry. Food Chemistry, 2008, 108, 310–315.
Shrivastava, A. & Gupta, V. B. Methods for the determination of limit of detection and limit of quantitation of the Analytical methods. Chronicles of Young Scientists, 2011, 2, 21-25.
Hecimovic, I., Cvitanovic, B. A., Horzic, D. & Komes. D. Comparative study of polyphenols and caffeine in different coffee varieties affected by the degree of roasting. Food Chemistry, 2011, 129, 991–1000.
Hagos Yisak, Mesfin Redi-Abshiro & Bhagwan Singh Chandravanshi. Selective determination of caffeine and trigonelline in aqueous extract of green coffee beans by FT-MIR-ATR spectroscopy. Vibrational Spectroscopy, 2018, 97, 33–38.
Gichimu, B. M., Gichuru, E. K., Mamati, G. & Nyende, A. B. Biochemical composition within Coffea arabica cv. Ruiru 11 and its relationship with cup quality. Journal of Food Research, 2014, 3, 31–44.
Sahar, A., Maryam, S. & Parisa, Z. Comparative Study of Mineral Elements and Caffeine in Imported Coffee Varieties Affected by the Degree of Roasting by HPLC Analysis. Journal of Chemical and Pharmaceutical Research, 2016, 8, 111-116.
Belete Tewabe Gebeyehu & Solomon Libsu Bikila. Determination of caffeine content and antioxidant activity of coffee. American Journal of Applied Chemistry, 2015, 3, 69-76.
Ephrem Demissie, Girma Woyessa & Arayaselassie Abebe. UV-Vis spectrophotometery determination of caffeine in green coffee beans from Hararghe, Ethiopia, using Beer-Lambert’s law and integrated absorption coefficient techniques. Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2016, 17, 109-123.
Maria, B. S., Paulo, M. & Marinez, A. L. Caffeine content of Ethiopian Coffea arabica beans. Genetics and Molecular Biology, 2000, 23, 213-215.
Legesse Adane, Mesfine Shiferaw & Israel Alemayehu. Determination of Caffeine Content of Bale Coffee Using HPLC Analysis. Food Science and Quality Management, 2018, 73, 23-32.
Dessalegn, Y., Labuscagne, M. T., Osthoff, G. & Herselman, L. Variation of green bean caffeine, chlorogenic acids, sucrose and trigolline contents among Ethiopian Arabica coffee accessions. Ethiopian Journal of Science, 2008, 30, 77–82.
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