The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm
Kouam Foubi Brice Bertrand,
Chuisseu Djamen Dieudonné Pascal,
Dzeufiet Djomeni Paul Désiré,
Samba Ngano Odette,
Moifo Boniface,
Zeh Odile Fernande,
Guegang Goujou Emilienne,
Mbede Maggy,
Tiedeu Alain Bertin,
Gonsu Fotsin Joseph
Issue:
Volume 1, Issue 2, December 2016
Pages:
36-41
Received:
22 October 2016
Accepted:
23 November 2016
Published:
5 January 2017
DOI:
10.11648/j.ijbbmb.20160102.11
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Abstract: Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.
Abstract: Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolec...
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Magnetic Water as Synergist of Pesticides Against Monacha cartusiana
Issue:
Volume 1, Issue 2, December 2016
Pages:
42-45
Received:
31 October 2016
Accepted:
24 November 2016
Published:
5 January 2017
DOI:
10.11648/j.ijbbmb.20160102.12
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Abstract: Magnetic water was used as an attractive reagent of snails, Monacha cartusiana, to pick more pesticides namely Thiamethoxam and Diafenthiuron, under laboratory conditions for 24 hours. Toxicity, synergism and food consumption rate were assessed in the presence and absence of magnetic water. Without magnetic water Thiamethoxam showed higher activity with LC50 (5300 μl/l) than Diafenthiuron LC50 (6500 μl/l). With magnetic water, the toxicity clearly increased as the LC50s observed, were 1900 and 3300 μl/l of Thiamethoxam and Diafenthiuron, respectively, with synergism ratios 2.79 and 1.97% .Results revealed that mixtures of molluscicides and magnetic water could increase toxicity against M. cartusiana effectively.
Abstract: Magnetic water was used as an attractive reagent of snails, Monacha cartusiana, to pick more pesticides namely Thiamethoxam and Diafenthiuron, under laboratory conditions for 24 hours. Toxicity, synergism and food consumption rate were assessed in the presence and absence of magnetic water. Without magnetic water Thiamethoxam showed higher activity...
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Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria
Bello Ibrahim,
Abdulsalami Mohammed Sani,
Bulus Timothy,
Mukhtar Gambo Lawal,
Abdullahi Mohammed,
Abdurrahman Siraj Abubakar,
Ahmad Mohammad
Issue:
Volume 1, Issue 2, December 2016
Pages:
46-49
Received:
18 November 2016
Accepted:
7 January 2017
Published:
3 February 2017
DOI:
10.11648/j.ijbbmb.20160102.13
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Abstract: G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.
Abstract: G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with...
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